Cajal's gold sublimate
WebA Modification for Paraffin Sections of the Cajal Gold-Sublimate Stain for Astrocytes * Julia Naoumenko, Julia Naoumenko New York, N. Y. Search for other works by this author on: Oxford Academic. PubMed. Google Scholar. Irwin Feigin, M.D. Irwin Feigin, M.D. New … WebPrinicple - astrocytes are selectively stained w/ Cajal gold sublimate method on frozen sections: Cajal stain: fix - formalin ammonium bromide for no less than 2days but no more than 25 days: cajal stain: section - frozen section cut at 20-30microns, tissue is free floated not placed on slides: Cajal stain: Control - cerebral cortex not spinal ...
Cajal's gold sublimate
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WebTHE CORRECT ANSWER IS "A." Cajal's gold sublimate method is used to demonstrate astrocytes in frozen tissue sections that have been fixed in formalin ammonium bromide solution. A modified phosphotungstic acid-hematoxylin procedure can be used to demonstrate: a. endothelial cells b. glial fibers WebA modification of Cajal's gold-sublimate method for tissue fixed in formalin Acta Neuropathol. 1966 Jan 14;6(1):88-92. doi: 10.1007/BF00691085.
WebAstrocytes selectively stained in frozen sections using Cajal gold sublimate method; Cajal Method: Fixative. Formalin ammonium bromide; 2-25 days; IF previously fixed in NBF, use FAB for 48hrs ... Transfer to gold sublimate, in dark, 4hrs, dH2O; 5% Hypo 2min, dH2O; Mount, blot (bibulous paper), Dehydrate 95% EtOH; Clear in Xylene, mount ... WebA modification of Cajal's gold-sublimate method for tissue fixed in formalin. SummaryA modification of Cajal's astrocyte stain was developed and carefully standardized, …
Web[modification of cajal's gold sublimate method for paraffin sections] [modification of cajal's gold sublimate method for paraffin sections] acta neuropathol. 1963 jun 6;3:501-3. doi: 10.1007/bf00685761. [article in german] author c descalzo. pmid: 14196526 doi ... Webcut and then stained with Cajal’s gold sublimate method (3). In short: (a) wash sections well in distilled water; (b) stain for4hinadark place, in a gold sublimate solution (a mixture of gold chloride 1% and mercuric chloride 1%); (c) wash in distilled water; (d) fix for 2 min in a 5% sodium thiosulfate solution; (e) wash thoroughly in dis-
WebThe preferred fixative for sections to be stained by the Cajal technique is: a. formalin ammonium bromide. b. 10% NBF. c. 10% aqueous formalin. d. alcoholic formalin. a. …
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